The rabbit anti-acetyl lysine antibody is developed using acetylated KLH as the immunogen. It is then affinity purified with acetyl lysine agarose, followed by conjugation with fluorescein isothiocyanate (FITC).

Acetylation of lysine is an important, reversible modification. The activities of some proteins are controlled by acetylation of lysine. Histone acetyltransferases (HATs) acetylate the conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) that contain lysine residues. Histone deacetylases (HDACs) remove the acetyl group from the same residue. [1] Acetylation/deacetylation of histones results in cell signalling processes that include gene activity, cell growth, differentiation and apoptosis. [2] In cancer and polyglutamine diseases, the regulation of protein acetylation/deacetylation is impaired. [3] Numerous anti-cancer drugs target HDACs.

Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is six months from date of shipping if stored properly.

1.  Curr. Opin. Chem. Biol. 1997. 1(3): 300-308. doi:10.1016/S1367-5931(97)80066-X.
2.  Curr. Cancer Drug Targets. 2004. 4(2): 205-218.  doi:10.2174/1568009043481560.