Biotin Antibody Agarose(ICP0615)

Applications:
IP; Immunoaffinity chromatography;
Formulation:
1 mL beads in 2 mL slurry with 50% glycerol
Pack Size
Price (USD)
Quantity
10 mg
$950.00
In Stock Request a Bulk Order
  • Product Description

    The biotin antibody is developed using biotin-KLH conjugate and purified with biotin-agarose affinity chromatography. The purified biotin antibody is chemically linked to beaded agarose through a stable covalent linkage. The biotin antibody-agarose can be utilized for reversible immunoprecipitation and affinity purification of biotinylated proteins/peptides.
  • Scientific Description

    Biotin is a vitamin, which binds strongly and irreversibly to avidin. Biotin is commonly used for labelling reagents. Anti-biotin antibody binds to biotin strongly but reversibly, the binding of the biotinylated species could be competitively eluted with free biotin.

  • Antibody Immobilized

    10 mg/mL agarose

  • Storage & Stability

    Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Shake throughly before use. Expiration date is one year from date of shipping if properly stored.

  • Product Specific References

    1. Nature Methods. 2017. 14 (12): 1167-1170. 10.1038/nmeth.4465
    2. EMBO reports. 2019. 20 (1): 1-21. https://doi.org/10.15252/embr.201847192
    3. Biotechniques. 2019. doi.org/10.2144/btn-2019-0088 
    4. Science Signalling. 2020. 13(627). doi: 10.1126/scisignal.aba4200
    5. Nature Com., 2020 11(1), 3133. 10.1038/s41467-020-16929-8
    6. Cell Chemical Biology. 2021. S2451-9456(21)00442-6. doi: 10.1016/j.chembiol.2021.10.008
    7. Developmental Cell. 2021. 56(1):52-66.e7. doi: 10.1016/j.devcel.2020.12.014
    8. bioRxiv. 2021. 2021.06.17.448349. www.biorxiv.org/content/10.1101/2021.06.17.448349v1
    9. Cell Press. 2022. 185(4): 712-728.E14. doi: https://doi.org/10.1016/j.cell.2021.12.041
    10. Nature Communications. 2022. 13(610). doi: https://doi.org/10.1038/s41467-022-28139-5
    11. Journal of Cell Biology. 2022. 221 (8): e202110089. doi: https://doi.org/10.1083/jcb.202110089
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