The anti-phosphotyrosine antibody is developed using phosphotyrosine as the antigen and affinity-purified with phosphotyrosine agarose. The purified antibody is immobilized to beaded agarose via strong thiol-ether linkages. This product could be utilized as an affinity matrix for rapid isolation and purification of proteins or peptides with phosphotyrosine residues.

Protein phosphorylation is one of the most important events in post-translational modification. Protein tyrosine phosphorylation has been extensively studied and is believed to be one of the switches of cell signaling. Simple and effective tools for rapid isolation and purification of the phosphorylated species is essential for proteomic profiling of the phosphorylated proteins in different stages of development, disease, and signal transduction, as well as identifying the precise site of phosphorylation in a phospho-protein.

5 mg/mL; antibody is covalently linked to CL-4B sepharose

Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is one year from date of shipping if stored properly.