Mono-Methyl Arginine Antibody

Applications:
ELISA (1:2000); WB (1:1000); IP (5 µg/mg protein sample); IF (5 µg/mL); IHC (5 µg/mL)

Specificity:
The antibody recognizes free mono-methyl arginine and proteins with mono-methylated arginine residues. No cross reaction with either asymmetric or symmetric dimethyl arginine.

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Product Description

The antibody is raised from rabbits immunized with NG- mono-methyl arginine-KLH conjugate, and affinity purified with mono-methyl arginine-agarose. It can be used for detection, isolation and localization of proteins/peptides with NG-mono-methylated arginine residues.
Scientific Description

Arginine methylation is a very common post-translational modification that occurs predominantly in the nucleus. The main pool of proteins that are modified possess RNA binding properties. More broadly, arginine methylation has been implicated in a variety of cellular processes, including protein trafficking, signal transduction and transcriptional regulation.
Storage & Stability

Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is one year from the date of shipping if properly stored.
Product Specific References
1. Mol. Microbiol. 2007. 65 (3): 590-606, doi:10.1111/j.1365-2958.2007.05831.x
2. J. Proteome Res. 2013. 12 (9): 3884-3899, doi:10.1021/pr400556c
3. Proteomics. 2013. 13 (7): 1059-1064. doi:10.1002/pmic.201200477
4. Proteomics. 2015. 15 (18): 3209-3218. doi 10.1002/pmic.201500075
5. J. Neurosci. 2015. 35 (37): 12890-12902. doi: 10.1523/JNEUROSCI.0246-15.2015
6. Proteomics. 2016. 16 (3): 465-476. doi: 10.1002/pmic.201400564
7. Molecular Cell. 2021. doi: 10.1016/j.molcel.2021.06.004