The affinity purified rabbit anti-phosphotyrosine antibody was conjugated to d-biotin via N-acetylation. The conjugates are useful for the detection, identification, and quantization of tyrosine-phosphorylated proteins and peptides. These conjugates have been utilized for non-radioactive tyrosine kinase assays.

Threonine is one of the 20 naturally occurring amino acids used for the biosynthesis of proteins. Kinases phosphorylate tyrosine to form phosphotyrosine, which is considered a key step in the regulation of enzymatic activities such as signal transduction.

Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is one year from date of shipping if properly stored.

1. J. Bio. Chem. 2006. 281: 37877-37887. doi:10.1074/jbc.M609398200