Western blot analysis of the acetylated protein profile in HeLa cell lysate with anti-acetyl lysine antibodies (ICP0380, lane A) and with additional acetylated BSA (10 ug/mL) in the primary antibody (lane B)
DNA transcription cannot take place unless DNA is unwound from the nucleosomes. The cell unwinds the DNA by acetylating lysine residues within the proteins. It has been suggested that acetylation of non-histone proteins (e.g., transcription factors) and histones may be involved. Acetylation of these proteins may result in signal transduction within the chromatic domains.
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