Affinity purified rabbit polyclonal phosphoserine antibodies are developed using a phosphoserine-KLH conjugate and phosvitin mixture as the immunogen. The phosphoserine specific antibodies were affinity purified with serine-phosphorylated peptide immobilized on agarose. This affinity purified antibody showed higher affinity than phosphoserine antibodies from other sources.

The antibody recognizes proteins and peptides phosphorylated on serine residues. There are no cross reactions with phospho-tyrosine.

ELISA (1:250); WB (1:250); IP (10 µg/mg crude protein); IHC (1:50)

Serine is one of the 20 naturally occurring amino acids. Kinases phosphorylate serine to phospho-serine which is responsible for various signalling processes in eukaryotes.

Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is one year from date of shipping if stored properly.

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