Immunohistochemistry staining of human lung cancer with mouse anti-MLH1 using rabbit anti-mouse polymer (ICP9788) as secondary antibodies and followed by goat anti-rabbit IgG (Fab) HRP polymers (ICP9868) as the third antibodies.
Rabbit IgG Antibody, Fab(ICP2368)
Applications:
ELISA, WB, IHC
Specificity:
The antibody is specific against rabbit IgG, pre-adsorbed with human IgG
Rabbit IgG Antibody, Fab is prepared with immobilized papain (ICP1948) followed by affinity purification with Rabbit IgG on Agarose. The use of the Fab fragment for conjugates of enzymes may produce higher signal and lower background.
Scientific Description
Fab is a monomeric antigen binding domain of an immunoglobulin G (IgG). Digestion of the IgG will generate two monomers of Fab. The Fab is better than IgG whole molecules in terms of detection of antigen, particularly for the detection of human antigen as human cells may contain the Fc receptors.
Storage & Stability
Product is stable for 30 days at room temperature. For extended storage, store the product at -20°C. Expiration date is three years from the date of shipping if stored properly.