Phosphothreonine Antibody, Biotin(ICP9820)

Applications:
ELISA (0.25 - 1.0 µg/mL); WB (0.25 - 1.0 µg/mL)
Specificity:
The antibody is pan-specific against phospho-threonine. It cross reacts with phospho-serine slightly but does not cross-react with phospho-tyrosine.
Immunogen:
Phospho-threonine-KLH conjugates and phosvitin
Species:
Rabbit
Formulation:
250 µg/mL in PBS, 50% glycerol
Conjugation:
Acetylation of the primary amine with the active ester of biotin (NHS-activated d-biotin)
Pack Size
Price (USD)
Quantity
100 µg
$375.00
In Stock Request a Bulk Order
  • Product Description

    Affinity purified rabbit anti-phospho-threonine antibodies are conjugated to d-biotin via N-acetylation. The conjugates are useful for detection, identification and quantization of threonine-phosphorylated proteins and peptides.
  • Specificity

  • Applications

  • Scientific Description

    Threonine is one of the 20 naturally occurring amino acids used for the biosynthesis of proteins. Kinases phosphorylate threonine to form phospho-threonine which is considered a key step in the regulation of enzymatic activities such as signal transduction.

  • Storage & Stability

    Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is one year from date of shipping if properly stored.

  • Product Specific References

    1. 1. Betts, M. J. and Russell, R. B. (2003) Amino Acid Properties and Consequences of Substitutions, in Bioinformatics for Geneticists (eds M. R. Barnes and I. C. Gray), John Wiley & Sons, Ltd, Chichester, UK.doi: 10.1002/0470867302.ch14
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