Glutathione Antibody, HRP(CN1010)

Applications:
Quantitative ELISA; WB; IP (affinity chromatography); IF; IHC
Specificity:
This pan-specific antibody recognizes both GSH and GSSG, as well as glutathionylated proteins/peptides.
Immunogen:
Glutathione-KLH conjugate, linked through -S- bond
Species:
Rabbit
Formulation:
250 µg/mL in PBS with 50% glycerol
Conjugation:
Aldehyde-activated HRP via reductive amination HRP:antibody ratio = 3:1
Pack Size
Price (USD)
Quantity
50 µg
$250.00
In Stock Request a Bulk Order
  • Product Description

    This antibody is developed using glutathione (GSH)-KLH conjugate, and purified with antigen-specific affinity chromatography. The antibody is conjugated to horseradish peroxidase (HRP) via reductive amination. It can be utilized for detection and quantification of reduced (GSH) and oxidized (GSSG) glutathione, as well as glutathionylated proteins/peptides.
  • Scientific Description

    Glutathione is a tripeptide. It contains an unusual peptide linkage between the amine group of cysteine and the carboxyl group of the glutamate side chain. Glutathione, an antioxidant, protects cells from toxins such as free radicals. Glutathione can also modify proteins via disulfide bonds. Redox proteomics of glutathionylated proteins in oxidatively stressed human T lymphocytes have been reported.

  • Storage & Stability

    Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is six months from date of shipping if properly stored.

  • Product Specific References

    1. PNAS. 2005. 102(39): 13998-14003. doi:10.1073/pnas.0504398102
    2. J. Biol. Chem. 2007. 282(45): 32640-54. doi:10.1074/jbc.M702953200
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