The antibody recognizes proteins with trimethylation on their lysine residues (N-epsilon). There are no cross reactions with acetylated proteins or mono- and di-methylated proteins.
Immunogen:
Methylated KLH conjugates
Formulation:
250 µg/mL in PBS with 50% glycerol
Conjugation:
Flourescein 5(6) isothionate; FITC/Ab molar ratio - >20:1
This rabbit polyclonal antibody is raised with a chemically methylated protein antigen. The specific anti-tri-methyl-lysine antibody was affinity purified with trimethylated lysine antibodies and affinity adsorbed with N-(epsilon) mono- and di-methylated lysine. The purified anti-trimethyl-lysine antibody was labeled with FITC. The antibody could be utilized for detection and localization of proteins with N-trimethylated lysine residues.
Scientific Description
Trimethylation of the epsilon amino group of lysine residues in proteins is one of the most important events of post-translational modification.
Storage & Stability
Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is six months from date of shipping if stored properly.
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