Western blot analysis of phosphothreonine in SP2/0 myeloma cells activated with fetal bovine serum for 10 minutes after serum-free starvation overnight. Whole cell lysates (A) 12ul and (B) 4ul were immobilized and reacted with biotin-conjugated rabbit anti-phosphothreonine (ICP9820), and then detected with HRP-conjugated rabbit anti-Biotin (ICP0612) in the test.
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