The affinity purified rabbit polyclonal anti-methylated lysine antibody, biotin, is developed using a technique unique to ImmuneChem. The methylated lysine antibodies are affinity purified using N-methyl (epsilon amino group) lysine on agarose as the affinity matrix. The purified antibody was conjugated to d-biotin via acetylation. Direct labelling of the primary antibody will avoid the use of secondary antibodies therefore eliminating the interference of the secondary antibody conjugates.

MeK is a conserved post-translational modification which is an important biochemical process for many protein-protein interactions. It is found in many proteins, for example calmodulin, cytochrome B, chromosomal proteins, non-histones as well as neural storage body proteins. It has been suggested that methylation of lysine plays an important role in gene silencing. [1-3]

Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is one year from date of shipping if properly stored.

1. J. Biol. Chem. 2002.277: 34655-34657. doi:10.1074/jbc.C200433200
2. J. Biol. Chem. 2002. 277: 11621-11624. doi:10.1074/jbc.C200045200
3. J. Biol. Chem. 2003. 278:18346-18352. doi:10.1074/jbc.M300890200