Methylated (ε-N) Lysine Antibody(ICP0501)

Applications:
ELISA; WB; IHC
Specificity:
The antibody recognizes proteins or peptides methylated on lysine residues (mono, di-methyllysine). There are no cross reactions with acetylated proteins. The cross reactivity with tri-methyl lysine has not been tested.
Immunogen:
Methylated KLH conjugates
Species:
Rabbit
Formulation:
250 µg/mL in Tris-phosphate buffer, 50% glycerol
Pack Size
Price (USD)
Quantity
100 µg
$350.00
In Stock Request a Bulk Order
  • Product Description

    The affinity purified rabbit polyclonal anti-methylated lysine antibody was developed using a technique unique to ImmuneChem. The methylated lysine antibodies are affinity purified using N-methyl (epsilon amino group) lysine on agarose as the affinity matrix. The antibodies could be utilized for detection, quantization and isolation of proteins with methylated lysine residues.
  • Scientific Description

    MeK is a conserved post-translational modification and is an important biochemical process for many protein-protein interactions. It is found in many proteins, for example calmodulin, cytochrome C, chromosomal proteins, histones and non-histones as well as neural storage body proteins. It has been suggested that methylation of lysine plays an important role in gene silencing. [1-3]
  • Storage & Stability

    Store product at -20°C. Expiration date is one year from date of shipping if stored properly.

  • Product Specific References

    1. Biol. Chem. 2002.277: 34655-34657. doi:10.1074/jbc.C200433200
    2. Biol. Chem. 2002. 277: 11621-11624. doi:10.1074/jbc.C200045200
    3. J. Biol. Chem. 2003. 278: 18346-18352. doi.10.1074/jbc.M300890200
    4. Biochem & Biophys Res. Com. 451 (2): 229-234.    doi:10.1016/j.bbrc.2014.07.11
    5. Mol. BioSyst. 2013. 9: 2231-2247. doi: 10.1039/C3MB00009E
    6. Biochemical and Biophysical Research Communications Volume 451, Issue 2, 22 August 2014, Pages 229–234. doi: 10.1016/j.bbrc.2014.07.110.
    7. Proteomics. 2015. 15 (13): 2166–2176. doi: 10.1002/pmic.201400521
    8. Nature Communications. 2018. 9(1):3436. doi: 10.1038/s41467-018-05451-7
    9. BioRxiv 2018. doi: 10.1101/240952
    10. The FEBS Journal 2019. doi: 10.1111/febs.14867
    11. EMBO Reports. 2019. 20(5): e43260. doi: 10.15252/embr.201643260
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