The affinity purified rabbit polyclonal anti-methylated lysine antibody was developed using a technique unique to ImmuneChem. Our methylated lysine antibody is affinity purified using N-methyl (epsilon amino group) lysine on agarose as the affinity matrix. The antibodies could be utilized for detection, quantization and isolation of proteins with methylated lysine residues.

MeK is a conserved post-translational modification and is an important biochemical process for many protein-protein interactions. It is found in many proteins, for example calmodulin, cytochrome C, chromosomal proteins, histones and non-histones as well as neural storage body proteins. It has been suggested that methylation of lysine plays an important role in gene silencing. [1-3]

Store product at -20°C. Expiration date is one year from date of shipping if stored properly.

1. Biol. Chem. 2002.277: 34655-34657. doi:10.1074/jbc.C200433200
2. Biol. Chem. 2002. 277: 11621-11624. doi:10.1074/jbc.C200045200
3. J. Biol. Chem. 2003. 278: 18346-18352. doi.10.1074/jbc.M300890200
4. Biochem & Biophys Res. Com. 2014. 451 (2): 229-234.    doi:10.1016/j.bbrc.2014.07.11
5. Mol. BioSyst. 2013. 9: 2231-2247. doi: 10.1039/C3MB00009E
6. Proteomics. 2015. 15 (13): 2166–2176. doi: 10.1002/pmic.201400521
7. Nature Communications. 2018. 9(1):3436. doi: 10.1038/s41467-018-05451-7
8. BioRxiv. 2018. doi: 10.1101/240952
9. The FEBS Journal. 2019. doi: 10.1111/febs.14867
10. EMBO Reports. 2019. 20(5): e43260. doi: 10.15252/embr.201643260