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The enrichment of DYKDDDDK-tag protein (50 kDa) from cell lysate by IP with DYKDDDDK-tag Antibody Agarose (ICP1315), followed by WB analysis with DYKDDDDK-tag Antibody HRP (ICP0902)
1: Molecular marker
2-3: 10 µL of original cell lysate
4: 10 µL of cell lysate obsorbed with DYKDDDDK-tag Antibody Agarose
5: 10 µL of enriched DYKDDDDK-tag protein solution
The DYKDDDDK sequence has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the DYKDDDDK tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. DYKDDDDK-tag facilitates the detection, isolation, and purification of the proteins. Affinity chromatography is one of the most efficient and rapid methods to enrich and purify the DYKDDDDK-tag proteins.
10-20 mg antibody /mL agarose beads. Antibody is covalently linked to agarose beads via amide linkage.
Product is stable for at least 30 days at room temperature. For extended storage, store product at -20°C. Expiration date is one year from date of shipping if stored properly.
