The His-tag (HHHHHH) antibody is immobilized to agarose beads via amide linkage. The product can be utilized as an affinity matrix for rapid immunoprecipitation, enrichment, isolation and purification of recombinant His-tag proteins.

The His tag (HHHHHH) has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the His tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. Affinity chromatography is one of the most efficient and rapid methods to enrich and purify His-tag proteins.

10-20 mg of antibody /mL of agarose beads

Product is stable for at least 30 days at room temperature. For extended storage, store product at -20°C. Expiration date is one year from date of shipping if stored properly.