Western-blot of cell lysate with His-tag antibody, HRP (ICP0851). The His-tag protein (50 kDa) in the cell lysate was enriched by IP with anti-His-tag Agarose (ICP1310).

1: Molecular marker
2: 10 µL of original cell lysate
3: 10 µL of cell lysate after IP
4: 10 µL of enriched His-tag protein

His-tag Antibody(ICP0850)

Applications:
ELISA (0.25-1 µg/mL); IP ( 1-10 µg, based on the sample size) ; WB (0.25-1.0 µg/mL); IHC (5 µg/mL)
Specificity:
Specific to His-tag proteins/peptides
Immunogen:
Polyhistidine peptide (HHHHHH) - KLH conjugate
Species:
Rabbit
Formulation:
500 µg/mL in PBS, 50% glycerol
Pack Size
Price (USD)
Quantity
200 μg
$295.00
In Stock Request a Bulk Order
Datasheet

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  • Product Description

    The His-tag antibody is developed using polyhistidine peptide His-tag(HHHHHH)-KLH conjugate, and affinity-purified with the HHHHHH peptide-agarose. The antibody is useful for detection, affinity isolation, enrichment and localization of His-tag proteins.

  • Scientific Description

    Polyhistidine peptide is frequently used as an affinity tag to facilitate the isolation of the 6H-fusion proteins using a metal-chelate matrix.

  • Storage & Stability

    Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is one year from date of shipping if stored properly.

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