Western-blot of cell lysate with His-tag antibody HRP (ICP0851). The His-tag protein (50 kDa) in the cell lysate was enriched by IP with anti-His-tag Agarose (ICP1310).

1: Molecular marker
2: 10 µL of original cell lysate
3: 10 µL of cell lysate after IP
4: 10 µL of enriched His-tag protein

Immunohistochemistry analysis of intact CHO cells (A) and CHO cells expressing a Hig-tag protein (B)

The cells were fixed with 4% paraformaldehyde for 15 minutes, followed by incubation with 5 ug/mL of His-tag Antibody, HRP (ICP0851) for 1 hour at room temperature.