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The acetylated peptides eluted from acetyl-lysine-specific affinity chromatography (ICP0388).
Approximately 5 mg of trypsinated crude protein from TSA treated MMRU cells were loaded.
The maximum binding of acetylated BSA with ICP0388-2MG.
-50 µl of ICP0388-2MG were incubated with 1 mg of acetylate BSA in a 1 ml tube for 60 min.
– After washing with PBST 4 times, the bound acetylated BSA was eluted with 1 ml of 0.5 M HCl
-5 µl / lane of the eluted acetylated BSA was resolved by SDS-PAGE and blotted wih monoclonal Mouse anti-Acetylated Lysine (ICP0390)
Trimethyl Lysine Antibody, Agarose
( ICP0606) View Details
Acetyl Lysine Antibody
( ICP0380) View Details
Acetyl Lysine Antibody, Agarose -5mg
( ICP0388-5MG) View Details
Biotin Antibody Agarose
( ICP0615) View Details
Estradiol-HRP
( CN1056) View Details Methylated Lysine Antibody, Agarose
( ICP0588) View DetailsThe antibody selectively captures proteins/peptides with acetylated lysine residues (N-epsilon). No cross-reactions to methylated proteins/peptides.
Rapid isolation and purification of proteins/peptides with acetylated lysine residues from cell lysate or protease-digested mixtures.
Protein acetylation is a form of post-translational modification known to regulate many diverse biological processes. Detection, isolation and identification of acetylated proteins/peptides is essential in proteomic studies. Affinity chromatography is one of the most efficient and rapid methods to enrich and purify the acetylated species for further MS/MS identification.
4 mg/ml antibody is covalently linked through amide bonds with NHS activated-SMCC, which further conjugates to thiolated agarose beads via thiol ether bonds.
Product is stable for 30 days at room temperature. For extended storage, store product at -20°C. Shake thoroughly before use. Expiration date is one year from date of shipping if properly stored.
