Pan-Formylmethionine antibodies recognize free fMet or fMet residues within proteins. Formylmethionine (fMet) plays a crucial role in the protein synthesis of bacteria, mitochondria, and chloroplasts. Polymorphonuclear cells can bind proteins starting with fMet, and initiate the attraction of circulating leukocytes. These in turn can stimulate microbicidal activities such as phagocytosis and initiate acute inflammation responses. Blocking of the fMet signal to inflammatory cells with the anti-fMet antibodies may prevent the acute inflammation induced by large scale cell injury. Our mouse monoclonal antibody was cloned from mice immunized with N-DL-formylmethionine that linked through its carboxyl group to the primary amine of KLH. This antibody can be specifically inhibited by free N-formylmethionine in a competitive ELISA. Pan-Formylmethionine antibodies can similarly recognize both free fMet as well as fMet residues within proteins.

Product is stable for several weeks at ambient temperature. For extended storage, aliquot content, and store product at –20ºC.