Recognizes proteins methylated on lysine residues (mono and di-methyllysine). It does not cross react with acetylated proteins. The cross reactivity to tri-methyllysine has not been tested.
Immunogen:
Methylated KLH conjugates
Formulation:
250 µg/mL in PBS, 50% glycerol
Conjugation:
With fluorescein isothiocyanate (FITC) FITC/Ab molar ratio - 10:1
Affinity purified rabbit polyclonal anti-methylated lysine antibody, FITC, is developed using a methylated protein as the immunogen. The methylated lysine specific antibodies are affinity purified with N-e-mono-methyllysine and di-methyllysine on agarose as the affinity matrix. The purified antibody was conjugated to fluorescein isothiocyanate (FITC). The conjugated antibody was tested to localize methylated proteins.
Storage & Stability
Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is six months from date of shipping if properly stored.