Crosstide (PRAATF) is a substrate of the PKB (AKT) and SGK kinase families. This affinity purified rabbit polyclonal anti-pCrosstide antibody is developed with phosphorylated crosstide (PRAApTF) and absorbed with non-phosphorylated crosstide. The purified phospho-Crosstide antibody is then conjugated to peroxidase (HRP) via reductive amination. The antibody has been utilized for non-radioactive PKB and SGK kinase assays.

Crosstide is a synthetic peptide with the sequence (GRPRTSSFAEG). Kinases phosphorylate crosstide to form phospho-crosstide. [1-3]

Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is six months from date of shipping if properly stored.

1. Nature. 1995. 378: 785-789. doi:10.1038/378785a0
2. Biochem. J. 1996. 315: 709-713. doi:10.1042/bj3150709
3. EMBO J. 1996. 15(23): 6541-6551. doi:10.1002/j.1460-2075.1996.tb01045.x