All Antibodies Linked on the Agarose Matrices are Affinity Purified, Antigen-Specific, and Covalently Linked to Agarose Beads with Stable Chemistry.

• Anti-Acetyl Lysine Agarose (ICP0388-5mg), (ICP0388-2mg)

Efficiently purify and enrich proteins or peptides with acetylated lysine-residues using our anti-acetyl lysine agarose product. This antibody has been widely used and documented by scientists worldwide.

Acetylated protein signal as determined by ELISA titration (OD50 titer).
The acetylated proteins were eluted from 1 mL of Rabbit anti-Acetyl Lysine Agarose (ICP0388) and immobilized on a microplate, then followed by detection with Mouse anti-Acetyl Lysine HRP. Rabbit anti-Biotin Agarose (1 mL) was utilized as the negative control (ICP0615).

• Anti-Biotin Agarose (ICP0615)

  Proteins or peptides with biotinylated residues can be efficiently purified and enriched with our anti-biotin agarose product.

• Anti-Trimethyl Lysine Agarose (ICP0606)

This antibody is affinity purified with free trimethyl lysine on agarose and absorbed by free monomethyl lysine and dimethyl lysine on agarose. It is highly specific for the trimethyl lysine and recognizes proteins peptides with tri-methylated lysine residues. Use our anti-trimethyl lysine agarose to efficiently purify and enrich proteins or peptides with biotinylated residues.

Elution profile of the trimethyl lysine signal from the affinity column (anti-trimethyl lysine agarose, ICP0606).
The sample loaded is from the trypsinated mouse spleen extract.

• Anti-Phosphoserine Agarose 

Phosphoserine antibodies are raised from a protein-phosphoserine mimic and affinity purified by phosphoserine. The antibody is linked to agarose beads in high concentration (>20 mg/mL of the Agarose beads). The product can be used for the selective immunoprecipitation as well as affinity-enrichment of serine-phosphorylated proteins/peptides.

• Anti-Phosphotyrosine Agarose (ICP9804)

The phosphotyrosine antibody is raised from phosphotyrosine-KLH conjugates and affinity purified by phosphotyrosine. The antibody is linked to agarose beads in high concentration (>20 mg/mL of the Agarose beads). Use our anti-phosphotyrosine agarose for selective immunoprecipitation and affinity-enrichment of tyrosine-phosphorylated proteins and/or peptides.

Immunoprecipitated samples with anti-phosphotyrosine antibodies agarose (ICP9804).
Left: Mouse spleen lysate treated with complete adjuvant; Right: Mouse spleen lysate without any treatment.
Detection: Phospho-tyrosine antibody, HRP (ICP9902)

• Anti-Methylated Lysine Agarose (ICP0588)

The antibody is raised from methylated KLH and affinity purified by methylated BSA and linked to agarose beads in high concentration (>30 mg/mL of the Agarose beads). Our anti-methylayed lysine agarose product can be used for selective immunoprecipitation and affinity-enrichment of methylated proteins/peptides

• Anti-Carboxylmethyl Lysine Agarose (ICP2188)

Carboxylmethyl lysine is the end product of advanced glycation. The antibody is raised from carboxymethylated KLH and affinity purified by carboxylmethyl lysine on agarose. It is linked to agarose beads in high concentration (>15 mg/mL of the Agarose beads). Try out this product for use in selective immunoprecipitation and affinity-enrichment of proteins/peptides with carboxylmethyl lysine residue and free carboxylmethyl lysines.