Using Biotin Antibody Agarose (ICP0615) to Enrich Biotinylated Peptides Without Desalting
September 3rd, 2025
(G) Experimental schematic of the SCASP-Biotin method. Peptides generated by SCASP were enriched for biotinylated peptides using mono-avidin beads or anti-biotin antibody-conjugated beads. The biotinylated peptides were desalted and analyzed on the timsTOF Pro mass spectrometer.
(H) Numbers of identified biotinylated peptides. A total of 1 × 107 THP1 cells were labeled with Sulfo-NHS-LC-biotin, followed by SCASP digestion and enrichment of biotinylated peptides. MS data were searched by MSFragger, and the numbers of biotinylated peptides are shown.
Z.-P. Lin et al., in their study, aimed to find a way to enrich multiple post-translational modifications (PTMs) efficiently and improve methods for data-independent acquisition mass spectrometry (DIA-MS) from a single sample. They proposed a desalting-free method for enriching a variety of PTMs with an SDS-cyclodextrin-assisted sample preparation. Our Biotin Antibody Agarose product (ICP0615) was used for enriching biotinylated peptides without desalting in their experiments to demonstrate the efficacy and results of their method.
We thank the researchers for using our Biotin Antibody Agarose beads in their study and developing novel and efficient methods to further scientific progress in the field.
Z.-P. Lin et al., “Comprehensive PTM profiling with SCASP-PTM uncovers mechanisms of p62 degradation and ALDOA-mediated tumor progression,” Cell Reports, vol. 44, no. 4, Apr. 2025, doi: 10.1016/j.celrep.2025.115500.
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