ImmuneChem’s Acetyl Lysine Antibody (ICP0380) was used in immunoprecipitation and immunoblotting experiments to examine the presence and level of acetylation of Set2 in set2Δ, TAP-Set2, bre1Δ, and gcn5Δ strains during aging. The antibodies were diluted in a 1:1000 ratio to follow yeast cell extracts in Western blots.
Li. Y-M., Mei. Y-C., Liu. A-H., et al. Gcn5- and Bre1- mediated Set2 degradation promotes chronological aging of Saccharomyces cerevisiae. Cell Reports. 2023. 42(10): 113186.