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Using ImmuneChem’s Mono-Methyl Arginine Antibodies (ICP0801), scientists have demonstrated that arginine monomethylation regulates the MAVS-mediated antiviral response

In Molecular Cell, researchers used ImmuneChem’s Mono-Methyl Arginine Antibodies (ICP0801) to readily detect monomethylated arginine (MMA) in THP1 cells infected with Sendai (SeV) virus. This monomethylation was found to occur on R52 and diminishes its interactions with RIG-1 as well as the formation of aggregates.

Figure 1D. A confocal microscopy image of MMA (monomethylarginine proteins) in THP1 cells detected using ImmuneChem’s Mono-Methyl Arginine Antibody (ICP0801).

Zhu. J., Li. X., Cai. X., et al. Arginine monomethylation by PRMT7 controls MAVS-mediated antiviral innate immunity. Molecular Cell. 2021. 81: 3171-3186.

Methods:

The cells were fixed with 4% paraformaldehyde in PBS for 20 minutes, permeabilized with 0.1% Triton X-100 and blocked with 1% BSA. They were then stained with Mono-Methyl Arginine Antibody (ICP0801) and then incubated with a fluorescent-dye conjugated secondary antibody.