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Prepare your desired antibody Fab fragments simply by letting the antibodies pass through a Papain Agarose (ICP1948) column

Prepare your desired antibody Fab fragments simply by letting the antibodies pass through a Papain Agarose (ICP1948) column.

Procedure:

1. Pack a column with the Papain Agarose (ICP1948) matrix, and activate it by washing with pre-warmed cysteine buffer (37C)
2. Mix you antibodies with cysteine buffer and pre warm the sample to 37C Then, load the antibody solution on the column
3. Collect the flow-through fraction which comprises of the Fab and Fc antibody fragments
4. Remove the Fc fragments by a Protein A column to obtain the antibody Fab fragments, or directly purify the Fab fragments with an antigen-specific affinity column

One gram of purified Goat anti-mouse IgG (lane 1) was loaded onto the Papain Agarose column (10mL) at a speed of 0.5mL/min. The flow-through (lane 2) was subsequently affinity bound to a Mouse IgG Agarose column, and the pure Fab fragment (lane 3) was then purified.