These rabbit polyclonal phosphoserine antibodies are developed using a phosphoserine-KLH conjugate and phosvitin mixture as the immunogen and affinity purified with serine-phosphorylated peptide immobilized on agarose.
Western blot analysis of phosphorylated proteins with UV-treated mouse cell lysates using phosphoserine antibodies (ICP9806).
(Lanes A: Control; B-D: Treatment with increased UV dose; E: Phosphatase inhibitor treatment)
These rabbit polyclonal phosphothreonine antibodies (anti-pT) are developed using a phosphothreonine-KLH conjugate and phosvitin mixture as the immunogen. The phosphothreonine specific antibody was affinity purified with phosphothreonine immobilized on agarose.
Detection of a phosphorylated protein kinase with phosphothreonine antibody (ICP9807)
(Lane 1: Untreated kinase; Lane 2: Kinase treated with phosphatase PP1G; Lane 3: MW marker time course).
These phosphotyrosine antibodies are raised using immunogenic conjugate phospho-tyrosine linked to KLH. The pan-specific antibodies for phosphotyrosine were specifically and selectively isolated from the immune serum using a unique technique which ensures the antibody can be competitively displaced by free phosphotyrosine. This anti-phosphotyrosine (anti-pY) antibody recognizes phosphotyrosine residues on both proteins and peptides.
Immunohistochemistry analysis of human breast cancer with phosphotyrosine antibodies (ICP9805).