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Increased PGC-1α acetylation in the retinal pigment epithelium of age-related macular degeneration donor eyes

Scientists who published a paper in Nature Scientific Reports have discovered an increased PGC-1α acetylation in the retinal pigment epithelium of age-related macular degeneration donor eyes.

Zhang. M., Jiang. N., Chu. Y., et al. Dysregulated metabolic pathways in age-related macular degeneration. Scientific Reports. 2020. 10: 2464.

A representative image of immunoprecipitation (IP) with ImmuneChem’s anti-Acetyl Lysine Antibody, Agarose (ICP0388), followed by revealing with an anti-PGC-1α antibody. Western blot analyses showed increased PGC-1α acetylation in retinal pigment epithelium (RPE) of age-related macular degeneration (AMD) patients (n=4) as compared to normal RPE (n=6).

Method: 

The extracted protein samples were incubated with ImmuneChem’s anti-Acetyl Lysine Antibody, Agarose (ICP0388) overnight at 4 °C. Agarose beads were then washed three times in IP buffer followed by three times wash in TAE buffer. The beads were directly incubated with SDS-PAGE sample buffer for 5 minutes at 95 °C. The resulting supernatants were used for Western-blot analysis, revealed with anti-PGC-1α antibody to determine PGC-1α acetylated protein levels.