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ImmuneChem’s Methylated Lysine Antibody, HRP (ICP0502) was used to help determine the methylation levels of BRD4, a fundamental transcription coactivator, in the presence of SETD6

In the Sciences Advances article: “BRD4 methylation by the methyltransferase SETD6 regulates selective transcription to control mRNA translation”

ImmuneChem’s Methylated Lysine Antibody, HRP (ICP0502) was used to help determine the methylation levels of BRD4, a fundamental transcriptional coactivator, in the presence of SETD6.

Vershinin. Z., Feldman. M., Weil. L. E., et al. BRD4 methylation by the methyltransferase SETD6 regulates selective transcription to control mRNA translation. Science Advances. 2021. 7(22): eabf5374.

Western blot analysis using methylated lysine antibody, HRP determined that the methylation level of BRD4 on chromatin increased in the presence of SET6, helping the researchers collectively determine that SET6 directly binds and methylates BRD4 on chromatin (Fig 1C).