Phospho-Crosstide Antibody(ICP0190)

Applications:
ELISA (0.25 - 1.0 µg/mL); WB (0.25 - 1.0 µg/mL); Non-radioactive AKT and SGK kinase assay
Specificity:
The antibody only recognizes the phosphorylated crosstide, the product of SGK and PKB in a non-radioactive kinase assay. There are no cross reactions with the non-phosphorylated crosstide.
Immunogen:
Phospho-crosstide (PRAApTF)-KLH
Species:
Rabbit
Formulation:
250 µg/mL in PBS, 50% glycerol
Conjugation:
Acetylation of the primary amine with the active ester of biotin (NHS-activated d-biotin).
Pack Size
Price (USD)
Quantity
100 µg
$375.00
In Stock Request a Bulk Order
  • Product Description

    Crosstide (PRAATF) is a substrate of the PKB (AKT) and SGK kinase families. Affinity purified rabbit polyclonal anti-pCrosstide antibody is developed with phosphorylated crosstide (PRAApTF) and absorbed with non-phosphorylated crosstide. The purified antibody was conjugated to peroxidase (HRP) via reductive amination. The antibody has been utilized for non-radioactive PKB and SGK kinase assays.
  • Specificity

  • Applications

  • Scientific Description

    Crosstide is a synthetic peptide with the sequence (GRPRTSSFAEG). Kinases phosphorylate crosstide to form phospho-crosstide. [1-3]

  • Storage & Stability

    Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is one year from date of shipping if properly stored.

  • Product Specific References

    1. Nature.1995. 378: 785-789. doi:10.1038/378785a0
    2. Biochem. J.1996. 315: 709-713.
    3. EMBO J.1996. 15(23): 6541-6551.
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