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ImmuneChem’s ICP0611 was used as an indicator for biotinylation in HeLa cells

ImmuneChem’s Rabbit Anti-Biotin Antibody (ICP0611) and a secondary anti-rabbit Alexa Fluor Plus 488 (probe to biotin antibody) was used as an indicator for biotinylation in HeLa cells (Chen et al., 2022).

Figure 2. (F) Confocal images of HeLa cells expressing STIM1-OptoID-mCherry (with iLID-P2A-SspB inserted at G99/E100) in response to blue light stimulation with or without biotin treatment (100 μM). Rabbit anti-biotin antibody and a secondary anti-rabbit Alexa Fluor Plus 488 (probe to biotin antibody) was used as an indicator for biotinylation. Blue, nuclear staining with Hoechst 33342.

Chen. R., Zhang. N., Zhou. Y., et al. Optical Sensors and Actuators for Probing Proximity-Dependent Biotinylation in Living Cells. Front. Cell. Neurosci. 2022. 16: 801644.

The researchers from this study were able to “optimize an EGFP-tagged monomeric streptavidin (mSA2) to serve as a biotinylation biosensor, which permits real-time monitoring of protein biotinylation in living cells (Chen et al., 2022).”