This antibody is developed using glutathione (GSH)-KLH conjugate, and purified with antigen-specific affinity chromatography. The antibody can be utilized for detection and quantification of reduced (GSH) and oxidized (GSSG) glutathione, as well as glutathionylated proteins/peptides.

 

 

Rabbit

250 µg/mL in PBS with 50% glycerol

Glutathione-KLH conjugate linked through -S- bond

Antigen-specific affinity chromatography

The pan-specific antibodies recognize both GSH and GSSG, as well as glutathionylated proteins/peptides.

Quantitative ELISA; WB; IP (affinity chromatography); IF; IHC

Glutathione is a tripeptide. It contains an unusual peptide linkage between the amine group of cysteine and the carboxyl group of the glutamate side chain. Glutathione, an antioxidant, protects cells from toxins such as free radicals. Glutathione can also modify proteins via disulfide bonds. Redox proteomics of glutathionylated proteins in oxidatively stressed human T lymphocytes have been reported.

Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is one year from date of shipping if properly stored.