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Dimethyl (ε-N) Lysine Antibody, HRP

Catalog # Pack Size Price(USD)
ICP0702 100 μg $395.00

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Product Description
The anti-dimethyl lysine antibody is developed using dimethylated KLH as immunogen, and purified with dimethyl lysine-agarose affinity chromatography. The purified antibodies are conjugated to horse radish peroxidase (HRP). The antibody can be utilized for detection, quantification and isolation of proteins/peptides with dimethylated lysine residues.
 
 
Western blot analysis of di-methylated proteins from swine spleen cell lysate using Rabbit anti-Dimethyl Lysine-HRP (ICP0702) with (B) and without (A) methylated BSA. 
Species
Rabbit
Formulation
250 µg/mL in stabalizing buffer with 50% glycerol
Immunogen
Dimethylated KLH
Purification
Affinity purified with dimethyl(ε-N) lysine-agarose
Conjugation
Reductive amination. Antibody: HRP molar ratio = 1:2
Specificity
Specific to proteins/peptides with dimethyl lysine residues. No cross reaction with acetylated lysine. Cross reactivity to monomethyl lysine not tested.
Applications
ELISA, WB
Scientific Description
Protein lysine methylation is a conserved post-translational modification and is an important biochemical process for many protein-protein interactions. It is found in many proteins, for example, calmodulin, cytochrome C, chromosomal proteins, histones, non-histones as well as neural storage body proteins. It has been suggested that dimethylation of lysine plays an important role in gene silencing.[1]
Storage & Stability
Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is six months from date of shipping if stored properly. Do not aliquot. Shake thoroughly before use.

References

 1. Mol. BioSyst. 2013. 9: 2231-2247. doi: 10.1039/C3MB00009E