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Dimethyl (ε-N) Lysine Antibody
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Product Description
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The anti-dimethyl lysine antibody is developed using dimethylated KLH as immunogen, and purified with dimethyl lysine-agarose affinity chromatography. The antibody can be utilized for detection, quantification and isolation of proteins/peptides with dimethylated lysine residues.
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![]() Western blot analysis of the di-methylated proteins from swine spleen with Rabbit anti-Dimethyl Lysine Antibody (ICP0701). Lane 2-3, acid-extraction histones; Lane 4-5, cells lysate
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Species
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Rabbit
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Formulation
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250 µg/mL in PBS, 50% glycerol
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Immunogen
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Dimethylated KLH
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Purification
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Affinity purified with dimethyl(ε-N) lysine-agarose
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Specificity
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Specific to proteins/peptides with dimethyl lysine residues. No cross reaction with acetylated lysine. Cross reactivity to monomethyl lysine not tested.
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Applications
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ELISA, WB, IP
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Scientific Description
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Protein lysine methylation is a conserved post-translational modification and is an important biochemical process for many protein-protein interactions. It is found in many proteins, for example calmodulin, cytochrome C, chromosomal proteins, histones, non-histones as well as neural storage body proteins. It has been suggested that dimethylation of lysine plays an important role in gene silencing.[1]
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Storage & Stability
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Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is three years from date of shipping if stored properly.
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References |
1. Mol. BioSyst. 2013. 9: 2231-2247. doi: 10.1039/C3MB00009E |