The affinity purified rabbit polyclonal anti-Di-methylated lysine antibodies are developed using a technique unique to ImmuneChem. The Di-methylated lysine antibodies are affinity purified using N-Di-methyl (epsilon amino group) lysine on agarose as the affinity matrix. The antibodies could be utilized for detection, quantization and isolation of proteins with Di-methylated lysine residues.

 

 

Western blot analysis of the di-methylated proteins from swine spleen cells lysate (lanes 4-5) and acid-extraction histones. (Lanes 2-3) with rabbit anti-di-methyl lysine antibodies (anti-MeK, ICP0701).

Rabbit

250 µg/mL in Tris-phosphate buffer, 50% glycerol

Di-methylated KLH conjugates

The antibody is  immunoaffinity purified with epsilon N-Di-methyl lysine on agarose.

The antibody recognizes proteins or peptides with di-methyl lysine residues. There is no cross reaction with acetylated proteins. The cross reactivity with mono-methyl lysine has not been tested.

ELISA, WB, IP

Protein lysine methylation (MeK) is a conserved post-translational modification and is an important biochemical process for many protein-protein interactions. It is found in many proteins, for example calmodulin, cytochrome C, chromosomal proteins, histones, non-histones as well as neural storage body proteins. It has been suggested that di-methylation of lysine plays an important role in gene silencing.^[1]

Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is three years from date of shipping if stored properly. Do not aliquot. Shake thoroughly before use.