The anti-dimethyl lysine antibody is developed using dimethylated KLH as immunogen, and purified with dimethyl lysine-agarose affinity chromatography. The antibody can be utilized for detection, quantification and isolation of proteins/peptides with dimethylated lysine residues.

 

 

Western blot analysis of the di-methylated proteins from swine spleen with Rabbit anti-Dimethyl Lysine Antibody (ICP0701). Lane 2-3, acid-extraction histones; Lane 4-5, cells lysate  

Rabbit

250 µg/mL in PBS, 50% glycerol

Dimethylated KLH 

Affinity purified with dimethyl(ε-N) lysine-agarose

Specific to proteins/peptides with dimethyl lysine residues. No cross reaction with acetylated lysine. Cross reactivity to monomethyl lysine not tested.

ELISA, WB, IP

Protein lysine methylation is a conserved post-translational modification and is an important biochemical process for many protein-protein interactions. It is found in many proteins, for example calmodulin, cytochrome C, chromosomal proteins, histones, non-histones as well as neural storage body proteins. It has been suggested that dimethylation of lysine plays an important role in gene silencing.^[1]

Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is three years from date of shipping if stored properly.