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Dimethyl (ε-N) Lysine Antibody

Catalog # Pack Size Price(USD)
ICP0701 100 μg $325.00

Quantity:

 

Product Description
The anti-dimethyl lysine antibody is developed using dimethylated KLH as immunogen, and purified with dimethyl lysine-agarose affinity chromatography. The antibody can be utilized for detection, quantification and isolation of proteins/peptides with dimethylated lysine residues.
 
 
Western blot analysis of the di-methylated proteins from swine spleen with Rabbit anti-Dimethyl Lysine Antibody (ICP0701). Lane 2-3, acid-extraction histones; Lane 4-5, cells lysate  
Species
Rabbit
Formulation
250 µg/mL in PBS, 50% glycerol
Immunogen
Dimethylated KLH 
Purification
Affinity purified with dimethyl(ε-N) lysine-agarose
Specificity
Specific to proteins/peptides with dimethyl lysine residues. No cross reaction with acetylated lysine. Cross reactivity to monomethyl lysine not tested.
Applications
ELISA, WB, IP
Scientific Description
Protein lysine methylation is a conserved post-translational modification and is an important biochemical process for many protein-protein interactions. It is found in many proteins, for example calmodulin, cytochrome C, chromosomal proteins, histones, non-histones as well as neural storage body proteins. It has been suggested that dimethylation of lysine plays an important role in gene silencing.[1]
Storage & Stability
Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is three years from date of shipping if stored properly. 

References

1. Mol. BioSyst. 2013. 9: 2231-2247. doi: 10.1039/C3MB00009E