This antibody is raised from rabbits immunized with N^G,N^G-dimethyl arginine-KLH, and affinity purified using the N^G,N^G-dimethyl arginine-agarose. The antibody is useful for detection, isolation and localization of proteins/peptides with asymmetric dimethylated arginine residues.

 

Western blot of human MMRU cells using Rabbit anti Dimethyl (asy) Arginine (ICP0810) with (C,D) and without (A,B)  free dimethyl arginine.

 

 

 

 

 

Rabbit

250 μg/mL in PBS, 50% glycerol

N^G,N^G-dimethyl arginine-KLH 

Affinity purified with N^G,N^G-dimethyl arginine-agarose and adsorbed with N^G-monomethyl arginine-agarose.

Specific to asymmetric dimethylated arginine residues.  No cross-reaction with acetylated or monomethylated proteins/peptides.

Human, mouse and pig

ELISA (1:2000); WB (1:1000); IP (5 µg/mg protein sample); IF (5 µg/mL); IHC (5 µg/mL)

Arginine methylation is a very common post-translational modification that occurs predominantly in the nucleus. The main pool of proteins that are modified possess RNA binding properties. More broadly, arginine methylation has been implicated in a variety of cellular processes, including protein trafficking, signal transduction and transcriptional regulation.

Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is one year from date of shipping if properly stored.