This antibody was raised from rabbits that were immunized with N^G,N^G-dimethyl arginine-KLH conjugates and specifically isolated and affinity purified using the N^G,N^G-dimethyl arginine antigen. The affinity purified methyl arginine antibody is useful for detection, isolation and localization of proteins with methylated arginine (asymmetric dimethylation) residues.

 

 

 

 

Rabbit

250μg/mL antibody stored in PBS, 50% glycerol

N^G,N^G-dimethyl arginine-KLH conjugates

Antigen-specific immunoaffinity purification with N^G,N^G-dimethyl arginine and adsorbed with N^G-monomethyl arginine

The antibody recognizes proteins with dimethylated arginine residues.  It does not cross-react with acetylated proteins or monomethylated proteins.

Human, mouse and pig

ELISA (1:2000); WB (1:1000); IP (5 µg/mg protein sample); IF (5 µg/mL); IHC (5 µg/mL)

Arginine methylation is a very common post-translational modification that occurs predominantly in the nucleus. The main pool of proteins that are modified possess RNA binding properties. More broadly, arginine methylation has been implicated in a variety of cellular processes, including protein trafficking, signal transduction and transcriptional regulation.

Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is one year from date of shipping if properly stored.