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Dimethyl (asy) Arginine Antibody
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Product Description
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This antibody is raised from rabbits immunized with NG,NG-dimethyl arginine-KLH, and affinity purified using the NG,NG-dimethyl arginine-agarose. The antibody is useful for detection, isolation and localization of proteins/peptides with asymmetric dimethylated arginine residues.
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![]() Western blot of human MMRU cells using Rabbit anti Dimethyl (asy) Arginine (ICP0810) with (C,D) and without (A,B) free dimethyl arginine.
![]() Antigen Structure
![]() IF Staining of MMRU cells
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Species
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Rabbit
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Formulation
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250 μg/mL in PBS, 50% glycerol
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Immunogen
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NG,NG-dimethyl arginine-KLH
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Purification
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Affinity purified with NG,NG-dimethyl arginine-agarose and adsorbed with NG-monomethyl arginine-agarose.
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Specificity
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Specific to asymmetric dimethylated arginine residues. No cross-reaction with acetylated or monomethylated proteins/peptides.
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Species Tested
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Human, mouse and pig
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Applications
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ELISA (1:2000); WB (1:1000); IP (5 µg/mg protein sample); IF (5 µg/mL); IHC (5 µg/mL)
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Scientific Description
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Arginine methylation is a very common post-translational modification that occurs predominantly in the nucleus. The main pool of proteins that are modified possess RNA binding properties. More broadly, arginine methylation has been implicated in a variety of cellular processes, including protein trafficking, signal transduction and transcriptional regulation.
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Storage & Stability
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Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is one year from date of shipping if properly stored.
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Product Specific References |
1. J. Proteome Res. 2013. 12 (9): 3884-3899. doi:10.1021/pr400556c
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