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Dimethyl (asy) Arginine Antibody

Catalog # Pack Size Price(USD)
ICP0810 100 μg $325.00

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Product Description
This antibody is raised from rabbits immunized with NG,NG-dimethyl arginine-KLH, and affinity purified using the NG,NG-dimethyl arginine-agarose. The antibody is useful for detection, isolation and localization of proteins/peptides with asymmetric dimethylated arginine residues.
 
Western blot of human MMRU cells using Rabbit anti Dimethyl (asy) Arginine (ICP0810) with (C,D) and without (A,B)  free dimethyl arginine.
 
 
Antigen Structure
 
 
[Exposure: 10 Milliseconds]
IF Staining of MMRU cells
 
Species
Rabbit
Formulation
250 μg/mL in PBS, 50% glycerol
Immunogen
NG,NG-dimethyl arginine-KLH 
Purification
Affinity purified with NG,NG-dimethyl arginine-agarose and adsorbed with NG-monomethyl arginine-agarose.
Specificity
Specific to asymmetric dimethylated arginine residues.  No cross-reaction with acetylated or monomethylated proteins/peptides.
Species Tested 
Human, mouse and pig
Applications
ELISA (1:2000); WB (1:1000); IP (5 µg/mg protein sample); IF (5 µg/mL); IHC (5 µg/mL)
Scientific Description
Arginine methylation is a very common post-translational modification that occurs predominantly in the nucleus. The main pool of proteins that are modified possess RNA binding properties. More broadly, arginine methylation has been implicated in a variety of cellular processes, including protein trafficking, signal transduction and transcriptional regulation.
Storage & Stability
Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is one year from date of shipping if properly stored.

Product Specific References

 

1. J. Proteome Res. 2013. 12 (9): 3884-3899. doi:10.1021/pr400556c
2. Proteomics. 2016. 16 (3): 465-476. doi: 10.1002/pmic.201400564
3. Proteomics2013. 13 (7): 1059-1064. doi: 10.1002/pmic.201200477
4. Proteomics 2015. 15 (18): 3209-3218. doi: 10.1002/pmic.201500075
5. J. Neurosci. 2015. 35 (37): 12890-12902. doi: 10.1523/JNEUROSCI.0246-15.2015
6. The FEBS Journalhttps://doi.org/10.1111/febs.15057