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Acetyl Lysine Antibody, FITC

Catalog # Pack Size Price(USD)
ICP0385 100 µg $375.00

Quantity:

Product Description
The rabbit anti-acetyl lysine antibody is developed using acetylated KLH as the immunogen. It is affinity purified with acetyl lysine agarose, followed by conjugation with fluorescein isothiocyanate.
 
Immunoflourescent staining of TSA-treated and non-treated melanoma cells.
Species
Rabbit
Formulation
250 µg/mL in PBS (pH 7) with 50% glycerol
Immunogen
Acetylated KLH conjugates
Purification
Affinity chromatography with acetyl-lysine agarose
Conjugation
FITC:antibody = 10:1
Applications
For direct immunofluorescence experiments
Scientific Description
Acetylation of lysine is an important, reversible modification. The activities of some proteins are controlled by acetylation of lysine. Histone acetyltransferases (HATs) acetylate the conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) that contain lysine residues. Histone deacetylases (HDACs) remove the acetyl group from the same residue. [1] Acetylation/deacetylation of histones results in cell signalling processes that include gene activity, cell growth, differentiation and apoptosis. [2] In cancer and polyglutamine diseases, the regulation of protein acetylation/deacetylation is impaired (7). Numerous anti-cancer drugs target HDACs. [3]
Storage & Stability
Product is stable for several weeks at 4°C. For extended storage,  store product at -20°C.  Expiration date is six months from date of shipping if stored properly.

Product Specific References

  1.  1. Curr. Opin. Chem. Biol. 1997. 1(3): 300-308. doi:10.1016/S1367-5931(97)80066-X.
  2.  2. Curr. Cancer Drug Targets. 2004. 4(2): 205-218.  doi:10.2174/1568009043481560.