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Acetyl Lysine Antibody, Biotin

Catalog # Pack Size Price(USD)
ICP0383 100 µg $375.00

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Product Description
The affinity purified polyclonal rabbit  anti-acetylated lysine antibody is developed using acetylated KLH as the immunogen. The acetylated lysine-specific antibody is conjugated with d-Biotin via amine acylation. 
 
Western blot analysis of acetylated proteins from TSA treated (A) and non-treated (B) human melanoma cell (MMRU) lysates.
Species
Rabbit
Formulation
250 µg/mL antibody in PBS, pH 7, with 50% glycerol.
Immunogen
Acetylated KLH conjugate.
Purification
Affinity chromatography with acetyl-lysine agarose.
Conjugation
Acylation with NHS-biotin; biotin:Ab molar ratio = 10:1
Specificity
The antibody recognizes proteins acetylated on lysine residues. Tested: acetylated histone, acetylated BSA, and acetylated MBP. No cross-reaction to non-acetylated proteins.
Scientific Description
DNA transcription cannot take place unless DNA is unwound from the nucleosomes. The cell unwinds the DNA by acetylating the lysine residues within the proteins. It has been suggested that acetylation of non-histone proteins (e.g. transcription factors) and histones may be involved. Acetylation of the proteins may result in signal transduction within the chromatic domains.
Storage & Stability
Product is stable for several weeks at 4°C. For extended storage,  store product at -20°C. Expiration date is one year from date of shipping if stored properly.
Product Specific References
  1. 1. FEBS Lett. 2002. 513(1): 124-128. doi:10.1016/S0014-5793(01)03309-9.