The monoclonal antibody is developed with hybridoma technology using chloramphenicol-KLH as immunogen, and purified with chloramphenicol-agarose affinity chromatography. The antibody can be utilized for isolation, detection and quantification of chloramphenicol. 

Mouse

250 µg/mL in PBS containing 50% glycerol

Chloramphenicol-KLH 

Antigen-specific affinity chromatography

The antibody recognizes free chloramphenicol and its derivatives.

ELISA; WB; Affinity chromatography

Product is stable for several weeks at 4°C. For extended storage, aliquot and store product at –20ºC. Avoid cycles of freezing and thawing. Expiration date is one year from date of shipping if properly stored.

Chloramphenicol is a bacteriostatic antimicrobial agent originally derived from the bacterium Streptomyces venezuelae, isolated by David Gottlieb, and introduced into clinical practice in 1949. It is the first antibiotic to be manufactured synthetically on a large scale. Chloramphenicol is effective against a wide variety of microorganisms and is still very widely used in low income countries because of its extreme inexpensiveness. Detection and quantization of chloramphenicol in food/feed becomes increasingly common due to the concern of antibiotic abuse.