This antibody is developed using chloramphenicol-KLH as immunogen, and purified with chloramphenicol-agarose  affinity chromatography. The purified antibody is conjugated to horseradish peroxidase (HRP) through reductive amination. The product has been tested for application in direct-competitive ELISA for quantification of free chloramphenicol with a sensitivity of IC50 1 ng/mL under un-optimized conditions.

Rabbit

1 mg/mL in stabilizing buffer with 50% glycerol

Chloramphenicol-KLH

Antigen-specific affinity chromatography

Reductive amination. HRP: antibody ratio= 2: 1

The antibody recognizes free chloramphenicol and its derivatives

Direct competitive ELISA (0.25 - 1.0 µg/mL)

Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is six months from date of shipping if properly stored.